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The new COVID-19 rapid test technology performs PCR faster than similar tests on the market.

The new COVID-19 rapid test technology performs PCR faster than similar tests on the market.

Infrared heating of plasmonic nanoparticles facilitates multiple reverse transcriptase quantitative PCR for rapid detection of SARS-CoV-2. Credits: Abigail Ayers and Nicoletta Barolini / Columbia Engineering,

For over 30 years, the polymerase chain reaction (PCR) has been the gold standard for molecular diagnostic tests to detect genetic material such as viruses and human DNA. However, PCRs involving reverse transcription polymerase chain reaction (RT-PCR) are largely centralized rather than point-of-care (POC) settings because the equipment is bulky, expensive, and results take a long time. It takes place in a large laboratory. , And to do it, you need a trained technician. These limitations resulted in a lack of accurate POC diagnostics, and a bottleneck in test results, especially during the COVID-19 pandemic.

Researchers at Columbia Engineering and Rover Diagnostics today offer an RT-PCR platform that provides results comparable to longer laboratory-based tests, faster than other PCR tests on the market, in 23 minutes. Announced that it was built. It can be adapted for testing a wide range of infectious diseases, including not only COVID-19, but also influenza, streptococci, and other viruses that require prompt diagnosis. Its target sensitivity is higher than other types of tests such as isothermal, antigen, and CRISPR. Also, the Rover PCR, which weighs only £ 2, is easy to carry and can be used by anyone.

“Our goal was to create a platform for pharmacies, transportation hubs, public events, and companies screening employees to return to work, where rapid turnaround results are important. “Samsia, a professor of biomedical engineering, said. Associate Professor for Fourth Purpose and Strategic Impact in Colombia.

The system was co-developed with Rover Diagnostics, a biotechnology startup co-founded in 2018 by Sia, serial technology entrepreneur Mark Fasciano, and CEO of Rover. The platform uses sample preparation techniques developed in Sia’s lab to bypass the standard approach of Pelche devices that heat samples from the outside of the vial and combine a new approach to the thermal cycle. Instead, Rover’s system uses a photothermal process (plasmonic thermocycling) that relies on irradiated nanoparticles to generate heat rapidly from the inside.

The new COVID-19 rapid test technology performs PCR faster than similar tests on the market.

RT-qPCR sample-to-result workflow for rapid detection of SARS-CoV-2, including simple extraction-free sample preparation using custom cartridges. Results are achieved with 23 minutes of thermocycling, facilitated by plasmonics heating of gold nanoparticles and real-time multiple fluorescence monitoring. Credits: Abigail Ayers and Yuhang Hu / Columbia Engineering

The team successfully performed reverse transcriptase quantitative PCR (RT-qPCR) in a reaction vessel containing all PCR reagents. qPCR is the current gold standard testing technique for identifying COVID infections. While this technique provides quantification of infected units, it also presents many hurdles to the miniaturization of point of care (POC).

In the study published today Nature nanotechnologyResearchers have been able to take advantage of plasmonic nanoparticles (discrete metal particles that respond to infrared light by emitting heat) to enable real-time and multiplexed RT-qPCR in clinical specimens. Was dealt with.

“This should really move the needle in providing rapid and accurate molecular clinical diagnosis in a decentralized setting,” said Faciano, who turned computer scientists into software and biotechnology entrepreneurs. “We are now able to speed up thermal cycling, which is very important for testing DNA and RNA, and clinicians and patients do not have to wait too long for results as well.”

The Rover team is developing a commercial product that can detect COVID-19, its variants, and other infectious diseases.

Promising COVID-19 Rapid Testing Technique Enters Phase 1 of NIH Challenge

For more information:
Samuel Shea, Point of Care COVID-19 Multiple Reverse Transcriptase Quantitative Polymerase Chain Reaction Using Phrasmonic Nanoparticles for Diagnosis, Nature nanotechnology (2022). DOI: 10.1038 / s41565-022-01175-4.

Courtesy of Applied Sciences, Columbia University

Quote: New COVID-19 Rapid Testing Technology, a similar inspection of the market acquired from on July 25, 2022 (2022) Perform PCR faster than (July 25)-pcr-faster.html

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